Regulatory T cells (Tregs) play a pivotal role in maintaining immune homeostasis and preventing autoimmunity. Treg-based cell therapies have already shown efficient modulation of immune responses in several clinical settings. However, the complexity and the cost of manufacturing autologous cell products restricts their implementation to certain patient populations with the highest unmet need. Induced pluripotent stem cells (iPSCs) have emerged as suitable starting material for generating diverse cell types, including Tregs.
Results demonstrate the successful derivation of functional iPSC-Tregs exhibiting potent suppressive activity. Flow cytometry and epigenetic analyses confirm the identity of the Treg lineage, reinforcing the fidelity of our differentiation protocol. This study provides a robust platform for the derivation and functional characterization of iPSC-Tregs, paving the path towards their potential application in cell therapy against autoimmune, metabolic, and other inflammatory diseases.